Enzymatic synthesis of I-125/131 labeled 8-hydroxyquinoline glucuronide and in vitro/in vivo evaluation of biological influence
APPLIED RADIATION AND ISOTOPES, cilt.69, sa.2, ss.299-307, 2011 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 69 Sayı: 2
- Basım Tarihi: 2011
- Doi Numarası: 10.1016/j.apradiso.2010.10.027
- Dergi Adı: APPLIED RADIATION AND ISOTOPES
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.299-307
- Anahtar Kelimeler: 8-Hydroxyquinoline (8-OHQ), 8-Hydroxyquinolyl-glucuronide (8-OHQ-Glu), beta-Glucuronidase, UDP-glucuronyl transferase (UDPGT), glucuronid prodrugs, Iodine 125, Iodine 131
- Dokuz Eylül Üniversitesi Adresli: Evet
Özet
8-Hydroxyquinoline (8-OHQ) is a long-known molecule which due to its metal-complexation ability is frequently used for analysis. It is also called oxine. Oxine and derivatives have been investigated to process antitumor and antimicrobial activities. 8-Hydroxyquinolyl-glucuronide (8-OHQ-Glu) was enzymatically synthesized using microsome preparates separated from Hutu-80 cells, labeled with I-125 to perform a radionuclide labeled prodrug and investigated of its biological affinities on Hutu-80 (human duodenum intestinal adenocarcinoma), Caco-2 (human colorectal adenocarcinoma), Detroit 562 (human pharynx adenocarcinoma) cells and ACBRI 519 (primary human small intestine epithelial cells) in this work. UDP-glucuronyl transferase (UDPGT) rich microsome preparates, which are used for glucuronidation in enzymatic synthesis, were extracted from Hutu-80 cells.