THE DEVELOPMENT OF ALGINATE-BASED MATRICES LOADED WITH RIFAMPICIN FOR WOUND HEALING


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Bibire T., Ghiciuc C. M., Yılmaz C. N., Ursu R. G., Danila R.

Med. Surg. J. –Rev. Med. Chir. Soc. Med. Nat., Iaşi, vol.128, no.1, pp.167-176, 2024 (ESCI)

Abstract

THE DEVELOPMENT OF ALGINATE
-
BASED MATRICES LOADED WITH RIFAMPI
C-
IN FOR WOUND HEALING (Abstract): The proposed study aims to develop polymeric sy
s-
tems based on alginate and N
-
vinylcaprolactam (NVCL) coated with poly(D,L
-
lac
tide
-
co
-
glycolide) (PLGA) as vehicles for Rifampicin (RIF) as active substance. Rifampicin is an
agent used for tuberculosis treatment, but the present study proposes to be used for wound
healing. The proposed topic is a complex study comprising a lot of d
ata; herein information
on Rifampicin loaded matrix and its antimicrobial activity against the main strains of the fo
l-
lowing bacteria:
S. aureus
,
MRSA
,
E. coli
,
P. aeruginosa
.
Materials and methods
: Alginate
and NVCL were used as main materials for polymer preparation then coated with PLGA; the
polymeric matrix was loaded with Rifampicin as active substance. The preparation of pol
y-
mer consisted of various steps such as polymerization technique, wate
r in oil in water emu
l-
sification and then matrix lyophilization for characterization in solid state. Chromatography
was used to indicate the presence of the drug (TLC) and then HPLC was used to quantify the
amount of it. The antimicrobial activity was test
ed by culturing the tested substances against
some bacteria e.g.
S. aureus
,
MRSA
,
E. coli
,
P. aeruginosa
.
Results:
The tested matrix
showed positive results in terms of drug loading and antimicrobial activity and will be fu
r-
ther investigated for IC50 and c
ytotoxicity.
Conclusions
: Based on the obtained results, the
proposed rifampicin
-
loaded alginate
-
based matrix shows potential in wound treatment, i
n-
cluding surgical wound care.
Keywords
: RIFAMPICIN, ALGINATE MATRICES LOA
D-
ED, WOUND HEALINGTHE DEVELOPMENT OF ALGINATE
-
BASED MATRICES LOADED WITH RIFAMPI
C-
IN FOR WOUND HEALING (Abstract): The proposed study aims to develop polymeric sy
s-
tems based on alginate and N
-
vinylcaprolactam (NVCL) coated with poly(D,L
-
lac
tide
-
co
-
glycolide) (PLGA) as vehicles for Rifampicin (RIF) as active substance. Rifampicin is an
agent used for tuberculosis treatment, but the present study proposes to be used for wound
healing. The proposed topic is a complex study comprising a lot of d
ata; herein information
on Rifampicin loaded matrix and its antimicrobial activity against the main strains of the fo
l-
lowing bacteria:
S. aureus
,
MRSA
,
E. coli
,
P. aeruginosa
.
Materials and methods
: Alginate
and NVCL were used as main materials for polymer preparation then coated with PLGA; the
polymeric matrix was loaded with Rifampicin as active substance. The preparation of pol
y-
THE DEVELOPMENT OF ALGINATE
-
BASED MATRICES LOADED WITH RIFAMPI
C-
IN FOR WOUND HEALING (Abstract): The proposed study aims to develop polymeric sy
s-
tems based on alginate and N
-
vinylcaprolactam (NVCL) coated with poly(D,L
-
lac
tide
-
co
-
glycolide) (PLGA) as vehicles for Rifampicin (RIF) as active substance. Rifampicin is an
agent used for tuberculosis treatment, but the present study proposes to be used for wound
healing. The proposed topic is a complex study comprising a lot of d
ata; herein information
on Rifampicin loaded matrix and its antimicrobial activity against the main strains of the fo
l-
lowing bacteria:
S. aureus
,
MRSA
,
E. coli
,
P. aeruginosa
.
Materials and methods
: Alginate
and NVCL were used as main materials for polymer preparation then coated with PLGA; the
polymeric matrix was loaded with Rifampicin as active substance. The preparation of pol
y-
mer consisted of various steps such as polymerization technique, wate
r in oil in water emu
l-
sification and then matrix lyophilization for characterization in solid state. Chromatography
was used to indicate the presence of the drug (TLC) and then HPLC was used to quantify the
amount of it. The antimicrobial activity was test
ed by culturing the tested substances against
some bacteria e.g.
S. aureus
,
MRSA
,
E. coli
,
P. aeruginosa
.
Results:
The tested matrix
showed positive results in terms of drug loading and antimicrobial activity and will be fu
r-
ther investigated for IC50 and c
ytotoxicity.
Conclusions
: Based on the obtained results, the
proposed rifampicin
-
loaded alginate
-
based matrix shows potential in wound treatment, i
n-
cluding surgical wound care.
Keywords
: RIFAMPICIN, ALGINATE MATRICES LOA
D-
ED, WOUND HEALINGmer consisted of various steps such as polymerization technique, wate
r in oil in water emu
l-
sification and then matrix lyophilization for characterization in solid state. Chromatography
THE DEVELOPMENT OF ALGINATE
-
BASED MATRICES LOADED WITH RIFAMPI
C-
IN FOR WOUND HEALING (Abstract): The proposed study aims to develop polymeric sy
s-
tems based on alginate and N
-
vinylcaprolactam (NVCL) coated with poly(D,L
-
lac
tide
-
co
-
glycolide) (PLGA) as vehicles for Rifampicin (RIF) as active substance. Rifampicin is an
agent used for tuberculosis treatment, but the present study proposes to be used for wound
healing. The proposed topic is a complex study comprising a lot of d
ata; herein information
on Rifampicin loaded matrix and its antimicrobial activity against the main strains of the fo
l-
lowing bacteria:
S. aureus
,
MRSA
,
E. coli
,
P. aeruginosa
.
Materials and methods
: Alginate
and NVCL were used as main materials for polymer preparation then coated with PLGA; the
polymeric matrix was loaded with Rifampicin as active substance. The preparation of pol
y-
mer consisted of various steps such as polymerization technique, wate
r in oil in water emu
l-
sification and then matrix lyophilization for characterization in solid state. Chromatography
was used to indicate the presence of the drug (TLC) and then HPLC was used to quantify the
amount of it. The antimicrobial activity was test
ed by culturing the tested substances against
some bacteria e.g.
S. aureus
,
MRSA
,
E. coli
,
P. aeruginosa
.
Results:
The tested matrix
showed positive results in terms of drug loading and antimicrobial activity and will be fu
r-
ther investigated for IC50 and c
ytotoxicity.
Conclusions
: Based on the obtained results, the
proposed rifampicin
-
loaded alginate
-
based matrix shows potential in wound treatment, i
n-
cluding surgical wound care.
Keywords
: RIFAMPICIN, ALGINATE MATRICES LOA
D-
ED, WOUND HEALINGwas used to indicate the presence of the drug (TLC) and then HPLC was used to quantify the
amount of it. The antimicrobial activity was test
ed by culturing the tested substances against
some bacteria e.g.
S. aureus
,
MRSA
,
E. coli
,
P. aeruginosa
.
Results:
The tested matrix
showed positive results in terms of drug loading and antimicrobial activity and will be fu
r-
ther investigated for IC50 and c
ytotoxicity.
Conclusions
: Based on the obtained results, the
proposed rifampicin
-
loaded alginate
-
based matrix shows potential in wound treatment, i
n-
cluding surgical wound care.
Keywords
: RIFAMPICIN, ALGINATE MATRICES LOA
D-
ED, WOUND HEALING