The role of glutathione peroxidases and sister chromatid exchanges in squamous cell cancer of the larynx

YILDIZ T., Kuralay F., Vefa Yuceturk A., Solak M.

Journal of B.U.ON., vol.5, no.3, pp.307-312, 2000 (SCI-Expanded) identifier

  • Publication Type: Article / Article
  • Volume: 5 Issue: 3
  • Publication Date: 2000
  • Journal Name: Journal of B.U.ON.
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.307-312
  • Keywords: Glutathione peroxidases, Larynx cancer, Selenium, Sister chromatid exchange, Smoking, Squamous cell carcinoma
  • Dokuz Eylül University Affiliated: Yes


Purpose: The aim of the present study was to investigate the oxidative and cytogenetic changes in patients with squamous cell carcinoma of the larynx (SLC) and to compare the results taken with subjects who were chronic cigarette smokers or nonsmokers. Patients and methods: A total of 84 men were enrolled. Smokers (n=27) and nonsmokers (n=26) were considered as healthy control groups. The patients (n=31) were staged according to the 1992 AJCC staging system. In the study groups, both the total serum glutathione peroxidases (TSGPX) and selenium(Se)-dependent GPX (SSGPX) activities were determined in erythrocyte (μmol/min/gHb) and serum(U/L), using the Paglia and Valentine method. In the cytogenetic research, lymphocyte cell lines from venous blood were prepared for determining the ratios of sister chromatid exchanges (SCE). Results: Significant differences in the antioxidants system were observed between the patients with SLC and both of the control groups, suggesting that GPX activities in human blood are affected by cigarette smoking. Also, the ratio of SCE in SLC patients was significantly higher compared with nonsmokers (p<0.0001). Conclusion: The decreased level of antioxidant products is more pronounced in SLC and this is probably due to dietary problems or difficulties in swallowing resulting from the anatomical localization of this disease. Our results confirm that squamous cell carcinoma of the larynx related to smoking leads to decreased antioxidant activity and increased cytogenetic changes.