Isolation, Characterization and Antimicrobial Activity of Bacteriocin Producing Lactic Acid Bacteria from Raw Water Buffalo Milk

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Erbakan M., Özavcı V.

INSAC International Researches Congress on Natural and Engineering Sciences (INSAC-IRNES'23), Konya, Turkey, 18 - 19 March 2023, pp.201-202

  • Publication Type: Conference Paper / Summary Text
  • City: Konya
  • Country: Turkey
  • Page Numbers: pp.201-202
  • Dokuz Eylül University Affiliated: Yes


Antibiotic resistance is a globally emerging health care concern. In recent years, a major difficulty in treatment of diseases such as tuberculosis, malaria, pneumonia and certain nosocomial infections is the presence of multi drug-resistant pathogens. In this regard, availability of the alternative antimicrobial materials is of paramount importance. Bacteriocins are ribosome-derived antimicrobial peptides generated by vast majority of bacteria to gain advantage over their competition. Bacteriocins from lactic acid bacteria (LAB) are often employed as natural food preservatives in food industry to increase the shelf-life of products. Their utilization as antimicrobial and anti-cancer agents in medical and veterinary treatments have been proposed. Therefore, discovery of new bacteriocin-producer LAB strains will benefit health and food industry applications. In this study, bacteriocin producer LAB strains were isolated from water buffalo milk samples collected from dairy farms located in Sorgun and Akdağmağdeni districts of Yozgat Province. Selective culture media were used for isolation of LAB genera including Lactobacillus, Enterococcus and Bifidobacterium. Screening of bacteriocin producer strains were carried out by reverse-side agar spot test using E. coli and S. aureus as indicator bacteria. A further verification of bacteriocin activity was carried out using neutralized cell-free supernatants via agar well diffusion assay. LAB isolates were characterized using miscellaneous biochemical methods including TSI, MR-VP, indole and catalase tests. Majority of the bacteriocin producer strains formed some degree of inhibition zone against selected indicator bacteria implying a broad bacteriocin antimicrobial activity spectrum, which might enable their utilization as novel antimicrobial agents.