Biochemical composition, antimicrobial and antibiofilm activities of Syntrichia ruralis (Hedw.) F.Weber & D.Moh extracts against some resistant bacteria
Journal of Research in Pharmacy, cilt.30, sa.3, ss.743-755, 2026 (ESCI, Scopus, TRDizin)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 30 Sayı: 3
- Basım Tarihi: 2026
- Doi Numarası: 10.12991/jrespharm.1725436
- Dergi Adı: Journal of Research in Pharmacy
- Derginin Tarandığı İndeksler: Emerging Sources Citation Index (ESCI), Scopus, TR DİZİN (ULAKBİM)
- Sayfa Sayıları: ss.743-755
- Anahtar Kelimeler: antibiyofilm activity, antimicrobal activity, GC/MS, resistant bacteria, Syntrichia ruralis (Hedw.) F.Weber & D.Moh
- Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
- Dokuz Eylül Üniversitesi Adresli: Evet
Özet
This study investigates the biochemical composition, antimicrobial, and antibiofilm properties of Syntrichia ruralis (Hedw.) F.Weber & D.Moh extracts obtained using ethanol, methanol, n-hexane, and distilled water as solvents. Disk diffusion assays demonstrated selective antimicrobial activity against specific bacterial strains, including Staphylococcus epidermidis, Klebsiella pneumoniae, and Proteus vulgaris. The MIC value for S. epidermidis was notably low (155 µg/mL) with the methanol extract, while other strains exhibited MIC values exceeding 10,000 µg/mL. Antibiofilm assays revealed significant inhibitory effects, particularly against foodborne Listeria innocua and clinical Escherichia coli isolates, with inhibition rates ranging from 57% to 87%, comparable to the commercial disinfectant Halamid. In contrast, biofilm formation was enhanced in Listeria monocytogenes upon exposure to the extracts. The ethanol extract showed limited antioxidant activity in the DPPH assay (30.69% at 1000 µg/mL), with an EC₅₀ of 1743.47 µg/mL. GC-MS analysis identified a total of 50 compounds across all extracts. Palmitic acid, neophytadiene, vitamin E, and triterpenoids such as β-amyrin acetate were predominant in the ethanol extract, while the methanol and n-hexane extracts contained long-chain alkanes, sterols, and fatty acid derivatives. The presence of these compounds may underlie the observed biological effects. This study represents the first comprehensive report on the antimicrobial, antibiofilm, and antioxidant activities of S. ruralis and highlights its potential as a source of bioactive metabolites, especially for targeting biofilm-associated infections.