Research Information System
11.International Drug Chemistry Conference, Antalya, Turkey, 10 - 13 March 2023, pp.174
Turkey is country rich
in plants with both aromatic and medicinal properties. St. John's Wort is a
plant that grows naturally in Turkey and has a very high medicinal value [1].
Due to the secondary metabolites of St. John's Wort, its medicinal uses are
also quite high [2,3]. Therefore, in this study, it was aimed to analyze the
volatile components of the St. John's Wort plant grown in the Izmir region. St.
John's Wort was collected and dried from two different regions in Izmir, and
then the flower and leaf parts were separated and the volatile components were
analyzed qualitatively and semi-quantitatively by headspace solid phase
microextraction method. After extraction, volatile component analysis in flower and leaf parts
was performed by gas chromatography/ mass spectrometry (GC/MS). Divinylbenzene/ carboxen/ polydimethylsiloxane (DVB /
CAR / PDMS) fiber was used in headspace solid phase microextraction method.
Equilibrium time, extraction temperature and extraction time were optimized in
the headspace phase microextraction method. Optimum equilibrium time was 40
min, optimum extraction temperature was 80 °C and optimum extraction time was
10 min. Volatile components in the flower and leaf parts of St. John's Wort
collected from two different regions in Izmir were compared in terms of both
number and variety. For
identification of peaks, relative retention indices of C7-C40
n-alkanes (between 3.12-61.85 min) obtained with the same capillary column,
were compared with relative retention indices obtained from the libraries of
Wiley and NIST11 (National Institute of Standards and Technology as MS
library). Apart from terpene
compounds, various volatile compounds such as alcohol, carboxylic acid and
aldehyde were found.