Akademik Veri Yönetim Sistemi
49th FEBS Congress, İstanbul, Türkiye, 5 - 09 Temmuz 2025, cilt.15, sa.2, ss.478, (Özet Bildiri)
Adipogenesis is defined as the process of differentiation of preadipocytes to mature adipocytes. Inhibition of adipocyte differentiation can be used as a potential therapeutic strategy against adipogenesis and, thus, obesity. We aimed to investigate the adipogenesis process in widely used 3T3-L1 preadipocytes differentiated to adipocytes and to suppress the induced lipid accumulation with perfluorodecanoic acid (PFDA) during this differentiation process with curcumin (Cu.). PFDA, a plasticizing agent used in food containers to which humans may be exposed, has been observed to induce adipogenesis. Curcumin is known to have anti-adipogenic and anti-inflammatory properties. Cells were cultured with curcumin and PFDA separately or in combination. WST1 assay was used to determine cell viability. OilRedO staining was used to evaluate differentiation and intracellular lipid accumulation. Spectrophotometric measurement was performed for lipid quantification. Gene expression profiles of adipogenic genes PPARγ and C/EBPα were analyzed with RT-PCR. According to our WST1 results, high concentrations of curcumin and PFDA were observed to cause cell toxicity. According to the results of the OilRedO staining, Cu. doses that can suppress lipid accumulation on 3T3-L1 adipocytes were observed as 20, 25, 30, 35 and 40 μM, while PFDA doses that caused lipid increase were observed as 50, 75, and 100 μM. According to the gene expression experiment results, the Cu. doses that can suppress lipid accumulation on 3T3-L1 adipocytes were observed as 20 and 30 μM, while the PFDA doses that caused lipid increase were selected as 50 μM. However, it was observed that 30 μM curcumin dose was more effective than 20 μM. When used as a combination, 30 μM Cu.+50 μM PFDA showed more significant suppression than 20 μM Cu.+50 μM PFDA doses. In our preliminary results of RTPCR, anti-adipogenic effects of curcumin were detected, and PFDA has the potential to induce adipogenesis alone and in combination. *The authors marked with an asterisk equally contributed to the work.