Scabiosa atropurpurea: A rich source of iridoids with α-glucosidase inhibitory activity evaluated by in vitro and in silico studies

Kılınc H., Masullo M., Lauro G., D'Urso G., Alankus O., Bifulco G., ...More

Phytochemistry, vol.205, 2023 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 205
  • Publication Date: 2023
  • Doi Number: 10.1016/j.phytochem.2022.113471
  • Journal Name: Phytochemistry
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Analytical Abstracts, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, EMBASE, Food Science & Technology Abstracts, MEDLINE, Veterinary Science Database
  • Keywords: Caprifoliaceae, Iridoids, LC-ESI-FT-MS, Molecular docking, NMR analysis, Scabiosa atropurpurea, α-glucosidase inhibitory activity
  • Dokuz Eylül University Affiliated: Yes


Scabiosa atropurpurea L. subsp. maritima (L.) Arc. (Caprifoliaceae) is a plant widely distributed in the Mediterranean region and represented by 32 taxa in the flora of Turkey. In the present study, an in-depth phytochemical investigation of S. atropurpurea methanol extract of the whole plant was carried out using a combination of LC-ESI-FT-MS and NMR analysis. This approach allowed the isolation and structural elucidation of 28 compounds: 17 iridoids, 7 flavonoids, and 4 phenolic acids. Among these compounds, three previously unreported iridoids named secologanin-methyl-hemiacetal, atropurpurin A, and atropurpurin B were identified. The methanol extract of S. atropurpurea was assayed for its antioxidant and antihyperglycemic activity, showing a potent α-glucosidase inhibitory activity (IC50 = 100 μg/mL), higher than that exerted by acarbose (IC50 = 196 μg/mL), used as the positive control. Thus, the most abundant iridoids were selected to be tested for their antihyperglycemic activity, and molecular docking experiments were carried out to assess the possibility for selected compounds to form complexes with α-glucosidase enzyme active site.