Atıf İçin Kopyala
Beceriklisoy H., Schäfer‐Somi S., Kücükaslan I., Agaoglu R., Gültiken N., Ay S., ...Daha Fazla
REPRODUCTION IN DOMESTIC ANIMALS, cilt.44, sa.s2, ss.115-119, 2009 (SCI-Expanded)
-
Yayın Türü:
Makale / Tam Makale
-
Cilt numarası:
44
Sayı:
s2
-
Basım Tarihi:
2009
-
Doi Numarası:
10.1111/j.1439-0531.2009.01443.x
-
Dergi Adı:
REPRODUCTION IN DOMESTIC ANIMALS
-
Derginin Tarandığı İndeksler:
Science Citation Index Expanded (SCI-EXPANDED), Scopus
-
Sayfa Sayıları:
ss.115-119
-
Dokuz Eylül Üniversitesi Adresli:
Hayır
Özet
ContentsUterine tissue from pregnant bitches was investigated by qualitative RT‐PCR for the gene expression of local factors potentially important for the implantation of canine embryos. For this purpose, 10 bitches identified as being at the time of implantation or early placentation by means of ultrasonography before ovariohysterectomy (days 20–35, n = 10) provided tissues for comparison to tissue collected in a previous study and identified as early pregnant (n = 10) or non‐pregnant (n = 4) by embryo flushing after ovariohysterectomy (days 10–12 after mating; Schäfer‐Somi et al. 2008). Uterine tissue was excised from the middle of the left horn from early pregnant and non‐pregnant animals, including from interplacental and placentation sites. The following genes were investigated: CD‐4, ‐8; cyclooxygenase (COX)‐1, ‐2; granulocyte macrophage‐colony stimulating factor (GM‐CSF); hepatocyte growth factor (HGF); insulin‐like growth factor (IGF)‐1, ‐2; transforming growth factor (TGF) and tumour necrosis factor (TNF)‐α; interferon (IFN)‐γ; interleukin (IL)‐1β, ‐2, ‐4, ‐6, ‐8, ‐10, ‐12; leukaemia inhibitory factor (LIF) and leptin. Gene expression for CD‐8, COX‐1, TGF‐β, HGF, IGF‐1, IL‐2, ‐4,‐10, IFN‐γ and LIF were detected in the pre‐implantation uterus, and all except IL‐2 and ‐10 were still detectable during the implantation and placentation stage. During implantation, mRNA for IGF‐2 and GM‐CSF were additionally detected. The dioestrous uterus differed from the pregnant uterus because of the absence of CD‐8, IL‐4 and IFN‐γ and the expression of CD‐4, TNF‐α and IL‐6. The results suggest that IL‐4, IFN‐γ, CD‐8, GM‐CSF and IGF‐2 are regulated in a pregnancy‐specific manner and that GM‐CSF and IGF‐2 probably have growth supporting and immune modulating functions during implantation of the canine embryo.