Effect of long non-coding RNA linc00968 on apoptosis in in-vitro Alzheimer's model


Creative Commons License

Kurt S., Kızıldağ S.

The Injector, vol.2, no.3, pp.134, 2023 (Peer-Reviewed Journal)

  • Publication Type: Article / Abstract
  • Volume: 2 Issue: 3
  • Publication Date: 2023
  • Journal Name: The Injector
  • Page Numbers: pp.134
  • Dokuz Eylül University Affiliated: Yes

Abstract

Objective: Substantial evidence indicates that various Long Non-Coding RNAs (LncRNAs) are commonly involved in different neurodegenerative disorders. This study aimed to investigate the regulatory effect of LncRNA linc00968 on apoptosis in an in vitro model of Alzheimer's disease (AD).Methods: Ab 25-35 fragment was used in SH-SY5Y cells to mimic AD neurotoxicity. Cell viability was determined by MTT analysis and the inhibition concentration (IC50) value of Ab 25-35 was calculated as 40 μM. Expression of linc00968 in cells treated and untreated (control) with 40 μM Ab 25-35 was examined by quantitative real-time polymerase chain reaction (qRT-PCR). Then, linc00968 was transfected with siRNA into SH-SY5Y cells with and without Ab 25-35 to determine its cellular role on apoptosis, and its expression was confirmed by qRT-PCR. To examine their effects on apoptotic pathways, BCL-2, BAX, CYC-S and internal control GAPDH gene expressions were analyzed by qRT-PCR. In order to evaluate post-transcriptional effects, Bcl-2, Bax, Cyc-s and Beta actin levels were analyzed by western blot method.Results: Our results showed that LncRNA linc00968 deregulation is involved in apoptotic processes in neuroblastoma cells induced Alzheimer's neurotoxicity by Ab 25-35. Linc00968 silencing increased anti-apoptotic Bcl-2 expression, while decreased pro-apoptotic Bax and Cyc-s expressions and reversed Ab 25-35-induced neurotoxicity in SH-SY5Y cells.Conclusion: LncRNA linc00968 is involved in the cellular functions and biological processes of neuroblastoma cells in the in vitro AD model, and the diagnostic value and therapeutic potential of Linc00989 are highlighted in our study.Keywords: Amyloid beta, apoptosis, long non-coding RNA