Tissue Engineering of 3D Organotypic Microtissues by Acoustic Assembly

Zhu Y., Serpooshan V., Wu S., Demirci U., Chen P., GÜVEN S.

ORGANOIDS, vol.1576, pp.301-312, 2019 (SCI-Expanded, Scopus)

  • Publication Type: Article / Article
  • Volume: 1576
  • Publication Date: 2019
  • Doi Number: 10.1007/7651_2017_68
  • Journal Name: ORGANOIDS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.301-312
  • Keywords: 3D organotypic microtissues, Acoustic assembly, Acoustic waves, Cardiac scaffold, Cardiomyocyte, Cell assembly, Cell spheroids, In vitro tissue model, Regenerative medicine, Spheroid assembly, Tissue engineering
  • Dokuz Eylül University Affiliated: Yes

Abstract

There is a rapidly growing interest in generation of 3D organotypic microtissues with human physiologically relevant structure, function, and cell population in a wide range of applications including drug screening, in vitro physiological/pathological models, and regenerative medicine. Here, we provide a detailed procedure to generate structurally defined 3D organotypic microtissues from cells or cell spheroids using acoustic waves as a biocompatible and scaffold-free tissue engineering tool.