RNA’DAN VE PROTEOFORMLARA TEMEL ARAŞTIRMA YÖNTEMLERİ

Altun Z. S.

Klinisyenler İçin Temel Onkoloji, Safiye Aktaş,Mehmet Alakavuklar, Editör, Türkiye Klinikleri Yayınevi, Ankara, ss.102-105, 2021

  • Yayın Türü: Kitapta Bölüm / Mesleki Kitap
  • Basım Tarihi: 2021
  • Yayınevi: Türkiye Klinikleri Yayınevi
  • Basıldığı Şehir: Ankara
  • Sayfa Sayıları: ss.102-105
  • Editörler: Safiye Aktaş,Mehmet Alakavuklar, Editör
  • Dokuz Eylül Üniversitesi Adresli: Evet

Özet

In human cells, molecular events within our genetic information and its functional reflections take place between the DNA-RNA-Protein triangle. Many genetic and epigenetic changes occur in carcinogenesis and progression, especially mutations in DNA. Gene expression and microRNAs that are important in gene-mRNA expression and regulation; Next generation RNA sequencing, microarrays, and quantitative-Real-Time PCR methods are used. Basic oncologic research methods such as mass spectrometric proteomic analysis and western-blotting are used in the determination of protein expression. In order to reveal protein differences-oncoproteoforms in clinical oncology, the use of top-down proteomics and immunoprecipitation approaches is on the scientific schedule.