Basic Research Methods from RNA to Proteoforms


Altun Z. S.

in: BASIC ONCOLOGY FOR CLINICIANS, Safiye Aktaş,Mehmet Alakavuklar, Editor, Türkiye Klinikleri Yayınevi, Ankara, pp.102-105, 2021

  • Publication Type: Book Chapter / Chapter Vocational Book
  • Publication Date: 2021
  • Publisher: Türkiye Klinikleri Yayınevi
  • City: Ankara
  • Page Numbers: pp.102-105
  • Editors: Safiye Aktaş,Mehmet Alakavuklar, Editor
  • Dokuz Eylül University Affiliated: Yes

Abstract

In human cells, molecular events within our genetic information and its functional reflections take place between the DNA-RNA-Protein triangle. Many genetic and epigenetic changes occur in carcinogenesis and progression, especially mutations in DNA. Gene expression and microRNAs that are important in gene-mRNA expression and regulation; Next generation RNA sequencing, microarrays, and quantitative-Real-Time PCR methods are used. Basic oncologic research methods such as mass spectrometric proteomic analysis and western-blotting are used in the determination of protein expression. In order to reveal protein differences-oncoproteoforms in clinical oncology, the use of top-down proteomics and immunoprecipitation approaches is on the scientific schedule.