Akademik Veri Yönetim Sistemi
49th FEBS Congress, İstanbul, Türkiye, 5 - 09 Temmuz 2025, cilt.15, sa.2, ss.478-479, (Özet Bildiri)
Obesity is a global public health problem characterized by abnormal or excessive fat accumulation that may impair health. Preadipocytes transform into adipocytes by interacting with several transcription factors, such as PPARγ and C/EBPα and they are essential targets for obesity-related therapeutic approaches. Nutritional and medicinal substances like boric acid (BA) and calcium fructoborate (CaFB) may critically contribute to regulating adipogenesis and, thus, to treating obesity. CaFB is found in vegetables such as celery and broccoli, as well fruits such as grapes and plums. This study aimed to investigate the anti-adipogenic potentials of BA and CaFB by analyzing the gene expression levels of PPARγ and C/EBPα. 3T3-L1 preadipocyte cells were used as an in vitro culture model. Differentiation of preadipocytes to adipocytes was determined morphologically by OilRedO staining. The concentration of BA and CaFB was determined after finding IC50 doses by WST1 assay. RT-PCR analyses were performed for the expression of PPARγ and C/EBPα genes from mRNA after administration of 2 and 5 mM CaFB and 100 and 250 μg/mL BA to differentiate 3T3-L1 cells. ELISA assay was used for the measurement of PPARγ and C/EBPα protein expression. The expression of PPARγ and C/EBPα genes and protein levels were suppressed in boron-treated cells compared to the control. As far as we know, these results are the first to demonstrate the effects of CaFB on the mRNA and protein expression of PPARγ and C/EBPα in mouse adipocytes in vitro. CaFB administered at 5 mM was more efficient than BA for all-day intervals. This study demonstrated the anti-obesity effects of boron compounds by inhibiting lipid accumulation. Natural boron compounds may be alternatives in the fight against obesity without side effects. In this study, the beneficial impact of CaFB on the regulation of adipogenesis is reported, and the mechanisms underlying the anti-obesity effects of boron were provided. *The authors marked with an asterisk equally contributed to the work.