Determination of Tetracyclines in Milk, Eggs and Honey Using in-situ Ionic Liquid Based Dispersive Liquid-Liquid Microextraction

Kaynaker M., Antep M., MERDİVAN M.

JOURNAL OF ANALYTICAL CHEMISTRY, cilt.73, sa.1, ss.23-29, 2018 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 73 Sayı: 1
  • Basım Tarihi: 2018
  • Doi Numarası: 10.1134/s1061934818010070
  • Dergi Adı: JOURNAL OF ANALYTICAL CHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.23-29
  • Anahtar Kelimeler: tetracyclines, ionic liquid, in-situ dispersive liquid-liquid microextraction, high performance liquid chromatography, MASS-SPECTROMETRY, PYRETHROID PESTICIDES, CHROMATOGRAPHY, PERFORMANCE, EXTRACTION, ANTIBIOTICS, WATER, SAMPLES
  • Dokuz Eylül Üniversitesi Adresli: Evet

Özet

In this study, in-situ ionic liquid based dispersive liquid-liquid microextraction method for enrichment of tetracyclines before liquid chromatographic analysis has been improved. A 1-benzyl-3- methylimidazolium chloride was used as an ionic liquid. To increase extraction efficiency, some optimization parameters (amount of ammonium hexafluorophoshate, extraction time, centrifugation time, ratio of ionic liquid/salt) were investigated. At optimized conditions, enrichment factors of four tetracycline antibiotics (tetracycline, chlortetracycline, methacycline, doxycycline) were between 25 and 98. The residues of tetracyclines were not found in the studied real samples. For the accuracy of the method, the concentration of 50 and 250 mu g/L of standard tetracycline mixture solutions were spiked to the blank real milk, honey and egg samples and the percentage recoveries were obtained in the range of 75.8-109.7%.