Selecting housekeeping genes as references for the normalization of quantitative PCR data in breast cancer

Kilic Y., Celebiler A. C., Sakizli M.

CLINICAL & TRANSLATIONAL ONCOLOGY, vol.16, no.2, pp.184-190, 2014 (SCI-Expanded, Scopus) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 16 Issue: 2
  • Publication Date: 2014
  • Doi Number: 10.1007/s12094-013-1058-5
  • Journal Name: CLINICAL & TRANSLATIONAL ONCOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.184-190
  • Keywords: Breast neoplasms, Reverse transcriptase polymerase chain reaction, Real time polymerase chain reaction, Housekeeping genes, PUM1, RPL13A
  • Dokuz Eylül University Affiliated: Yes

Abstract

The common reference genes of choice in relative gene expression studies based on quantitative real time polymerase chain reaction, ACTB and B2M, were shown to be regulated differently in respect to tissue type. In this study, the stability of the selected housekeeping genes for normalizing the qPCR data were identified in the tumor and its adjacent tissues in invasive breast cancer, and the variability of their levels according to the stages and the histopathologic subtypes was analyzed.