An In-House Method for Molecular Monitoring of BCR-ABL


Sercan H. O., Ozturk I., Caliskan C., Pehlivan M., Sercan Z.

TURKISH JOURNAL OF HEMATOLOGY, vol.29, no.4, pp.348-353, 2012 (SCI-Expanded) identifier

  • Publication Type: Article / Article
  • Volume: 29 Issue: 4
  • Publication Date: 2012
  • Doi Number: 10.5505/tjh.2012.14892
  • Journal Name: TURKISH JOURNAL OF HEMATOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.348-353
  • Keywords: Chronic myeloid leukemia, BCR-ABL, Real-time PCR, Molecular response, HARMONIZING CURRENT METHODOLOGY, RESIDUAL DISEASE DETECTION, POLYMERASE-CHAIN-REACTION, CHRONIC MYELOID-LEUKEMIA, RT-PCR, STANDARDIZATION, TRANSCRIPTS, IMATINIB, RECOMMENDATIONS, RESPONSES
  • Dokuz Eylül University Affiliated: Yes

Abstract

Objective: At present, there are a limited number of facilities in Turkey that can provide reliable real-time quantitative (RQ)-PCR BCR-ABL results. The present study aimed to test a cost-effective, in-house method of BCR-ABL quantification, including verification of the method by RQ-PCR validation tests.