Rapid sensing of Cu+2 in water and biological samples by sensitive molecularly imprinted based plasmonic biosensor

Safran V., Gokturk I., Derazshamshir A., Yilmaz F., Sağlam N., Denizli A.

MICROCHEMICAL JOURNAL, vol.148, pp.141-150, 2019 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 148
  • Publication Date: 2019
  • Doi Number: 10.1016/j.microc.2019.04.069
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.141-150
  • Dokuz Eylül University Affiliated: No


In this study, copper (II) ion (Cu+2) imprinted poly(hydroxyethyl methacrylate-N-metacryloyl-(L)-cysteine methyl ester [PHEMAC-Cu+2] nanoparticles were synthesized by two-phase mini-emulsion polymerization method and applied to the SPR sensor chip surface for the selective determination of the Cu+2 ions in both aqueous solution, Cu+2-spiked artificial urine and physiological serum samples to investigate the effects of metabolite residues during the analysis. The non imprinted [PHEMAC] nanoparticles were synthesized by applying the same procedure for the [PHEMAC-Cu+2] nanoparticle synthesis except the addition of Cu+2 ions as a control experiment to evaluate the selectivity of the [PHEMAC-Cu+2] nanoparticles. Roughness differences between [PHEMAC-Cu+2] and [PHEMAC] nanoparticles showed that the imprinting process of Cu+2 ions was performed successfully. [PHEMAC-Cu+2] and [PHEMAC] SPR biosensors prepared by attaching nanoparticles onto the surface of sensor chips, were characterized by atomic force microscope, ellipsometer, contact angle measurements.