Synthesis and in vitro evaluation of 99mTc radiolabeled lapatinib (LPT) and its PLGA formulation

Gokulu S. G., Karatay K. B., Bilgi A., Kayas C., Yildirim N., Kilcar A. Y., ...More

Journal of Radioanalytical and Nuclear Chemistry, vol.333, no.2, pp.665-672, 2024 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 333 Issue: 2
  • Publication Date: 2024
  • Doi Number: 10.1007/s10967-023-09292-7
  • Journal Name: Journal of Radioanalytical and Nuclear Chemistry
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Analytical Abstracts, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Chimica, Communication Abstracts, Food Science & Technology Abstracts, INSPEC, Metadex, Pollution Abstracts, Public Affairs Index, Veterinary Science Database, Civil Engineering Abstracts
  • Page Numbers: pp.665-672
  • Keywords: Poly(lactic-co-glycolic acid) (PLGA), Lapatinib (LPT), Technetium-99 m (Tc-99m), Human breast adenocarcinoma (MDA-MB-231), Cervix adenocarcinoma (HeLa), Human ovarian (MDAH-2774)
  • Dokuz Eylül University Affiliated: Yes

Abstract

Lapatinib-(LPT) is a dual tyrosine kinase inhibitor that has important effects on the targeted therapy of various tumours. The current study is aimed to use a novel tracer strategy including Technetium-99 m-(99mTc) radiolabeled LPT and its poly(lactic-co-glycolic acid)-(PLGA) encapsulated formulation-(LPT-PLGA). In vitro bioaffinity of the [99mTc]Tc-LPT and [99mTc]Tc-LPT-PLGA was evaluated by using cell culture methods on human breast adenocarcinoma (MDA-MB-231), cervix adenocarcinoma and human ovarian (MDAH-2774) cancer cell lines. Radiochemical yield were over 95% and both compounds were examined high incorporation values on HeLa and MDAH cell lines. Current results may contribute to develope as the encapculated agents including radiotracer.