A highly sensitive and specific enzyme-linked immunosorbent assay of antibodies to hepatitis C virus


Eroglu C., Yildiz E., Ozturk M., Pinarbasi E.

ACTA VIROLOGICA, vol.44, no.1, pp.29-33, 2000 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 44 Issue: 1
  • Publication Date: 2000
  • Journal Name: ACTA VIROLOGICA
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.29-33
  • Keywords: hepatitis C virus, core protein gene, cloning, nucleotide sequencing, expression, E. coli, purification, ELISA, RT-PCR, CORE PROTEIN, POSTTRANSFUSION HEPATITIS, HCV INFECTION, BLOOD-DONORS, 1ST-GENERATION, EXPRESSION, DIAGNOSIS, ANTIGEN, GENOME
  • Dokuz Eylül University Affiliated: No

Abstract

In this study, a 178 amino acids long portion of the hepatitis C virus (HCV) core gene was cloned sequenced expressed in Escherichia coli, and purified. The resulting antigen (C178) was tested with human sera enzyme-linked immunosorbent assay (ELISA) in order to assess its ability to diagnose HCV. It was shown by ELISA that 92% of the patients sera, diagnosed previously by a 3(rd) generation enzyme immunoassay (ETA) as HCV-positive, had antibodies against the C178 antigen. This antigen gave no false positive results when tested with anti-HCV-negative sera.