A highly sensitive and specific enzyme-linked immunosorbent assay of antibodies to hepatitis C virus

Eroglu C., Yildiz E., Ozturk M., Pinarbasi E.

ACTA VIROLOGICA, cilt.44, sa.1, ss.29-33, 2000 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 44 Sayı: 1
  • Basım Tarihi: 2000
  • Dergi Adı: ACTA VIROLOGICA
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.29-33
  • Anahtar Kelimeler: hepatitis C virus, core protein gene, cloning, nucleotide sequencing, expression, E. coli, purification, ELISA, RT-PCR, CORE PROTEIN, POSTTRANSFUSION HEPATITIS, HCV INFECTION, BLOOD-DONORS, 1ST-GENERATION, EXPRESSION, DIAGNOSIS, ANTIGEN, GENOME
  • Dokuz Eylül Üniversitesi Adresli: Hayır

Özet

In this study, a 178 amino acids long portion of the hepatitis C virus (HCV) core gene was cloned sequenced expressed in Escherichia coli, and purified. The resulting antigen (C178) was tested with human sera enzyme-linked immunosorbent assay (ELISA) in order to assess its ability to diagnose HCV. It was shown by ELISA that 92% of the patients sera, diagnosed previously by a 3(rd) generation enzyme immunoassay (ETA) as HCV-positive, had antibodies against the C178 antigen. This antigen gave no false positive results when tested with anti-HCV-negative sera.