Akademik Veri Yönetim Sistemi
International Medical Journal, cilt.7, sa.3, ss.215-219, 2000 (Scopus)
Objective: In this study the effects of caffeic acid phenethyl ester (CAPE), antioxidant, were investigated on the nephropathy and antioxidant enzyme activities in kidneys treated with gentamicin. Design: This experimental study was designed to examine the protective effect of CAPE against nephrotoxicity in rats. Materials and Methods: Sixteen week-old male Sprague-Dawley rats were used in this study. There were 3 experimental groups: Group I, controls (n:8; injected with saline), group II, injected with gentamicin (n:8), group III, injected with gentamicin plus CAPE (n:8). Gentamicin was injected 100 mg/kg/d subcutaneously for 5 consecutive days. At the same time, CAPE was injected 10 μM/kg/d intraperitoneally for 5 days. After 24 h of the last injection, rats were sacrificed. The renal cortex was carefully separated from the medulla and homogenized. Blood samples were collected to determine the serum levels of urea, creatinine, Na+ and K+. Before sacrification, urine was collected for protein detection. The homogenized samples were centrifuged and the supernatants were used to determine Glutathione Peroxidase (GSH-Px), Catalase (CAT), and Superoxide Dismutase (SOD) activities. For histologic examination on light microscopy small sample from renal cortex was separated and placed in formaldehyde solution. Results were compared with Mann-Whitney U-test. Results: Gentamicin administration resulted in hyperproteinuria, increased γ-GT in serum, and a significant decrease in GSH-Px, CAT, and SOD activities in the kidneys compared to control. In the rats treated with gentamicin plus CAPE, there was a remarkable restoration in GSH-Px, CAT, and SOD activities as well as proteinuria and γ-GT compared to gentamicin treated group. There was no significant different in serum levels of urea, creatinine, Na+ and K+ among three groups. On the light microscopic examination, there was widest tubular necrosis varying degrees from grade 2 (G-2) to grade 4 (G-4) in gentamicin treated group. However, in rats treated with CAPE plus gentamicin there was a marked reduction in the extend of tubular damage. Conclusion: These results show that CAPE could protect kidneys against gentamicin induced tubular necrosis presumably because of antioxidant properties of CAPE, as evidenced by restoring SOD, CAT, and GSH-Px activities.