The role of neutrophils in the formation of peritoneal adhesions


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Vural B., Canturk N., Esen N., Solakoglu S., Canturk Z., Kirkali G., ...More

HUMAN REPRODUCTION, vol.14, no.1, pp.49-54, 1999 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 14 Issue: 1
  • Publication Date: 1999
  • Doi Number: 10.1093/humrep/14.1.49
  • Journal Name: HUMAN REPRODUCTION
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.49-54
  • Keywords: cyclophosphamide, granulocyte-macrophage colony-stimulating, factor, neutrophils, peritoneum, postoperative complications, GLYCOPROTEIN COMPLEX, MONOCLONAL-ANTIBODY, PREVENTION, CD18, MACROPHAGES, DECREASE, STRENGTH, COLLAGEN, RABBITS, INVIVO
  • Dokuz Eylül University Affiliated: No

Abstract

The most common cause of intraperitoneal adhesions which may result in infertility and intestinal obstruction is previous abdominal surgery. Surgical trauma of the peritoneum in the absence of infection elicits a rapid and transient influx of polymorphonuclear leukocytes (PMN) into the peritoneal cavity, The role of neutrophils in intraperitoneal adhesion formation has not been studied. We aimed to study the effects of PMN counts and PMN functions on peritoneal adhesion formation. Forty peritoneal adhesion-induced rats were randomly divided into three groups; group I, receiving saline; group LI, receiving cyclophosphamide; and group III, receiving granulocyte-macrophage colony-stimulating factor (GM-CSF), In all groups, peritoneal lavage was performed to determine PMN counts the day after adhesion induction. Blood neutrophil counts and neutrophil functions were also determined. Adhesions were evaluated blindly 14 days after the operation. Adhesion tissue samples were microscopically evaluated. Tissue hydroxyproline and collagen concentrations were measured. The neutrophil counts and phagocytosis significantly increased in group III and neutrophil counts decreased in group II (P < 0.05), The score of adhesion formation in group II was significantly less than that in groups I and III (P < 0.05). Hydroxyproline concentrations of adhesion tissue were significantly decreased in group II when compared with group III (P < 0.05). The present study shows that neutropenia lowers the degree of postoperative adhesion formation. It is concluded that PMN may have a role to play in modulating post-operative adhesion formation.