PRODUCTION OF LIGNINOLYTIC ENZYMES BY SOLID-STATE FERMENTATION USING Pleurotus eryngii

Akpinar M., ÖZTÜRK ÜREK R.

PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY, cilt.42, sa.6, ss.582-597, 2012 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 42 Sayı: 6
  • Basım Tarihi: 2012
  • Doi Numarası: 10.1080/10826068.2012.673528
  • Dergi Adı: PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.582-597
  • Anahtar Kelimeler: decolorization, grape waste, ligninolytic enzymes, Pleurotus eryngii, solid-state fermentation, ARYL-ALCOHOL OXIDASE, MANGANESE PEROXIDASE, LACCASE ISOENZYMES, DEGRADATION, CULTURES, WASTE, INDUCTION, OSTREATUS, OXIDATION, KINETICS
  • Dokuz Eylül Üniversitesi Adresli: Evet

Özet

Pleurotus eryngii (DC.) Gillet (MCC58) was investigated for its ability to produce various ligninolytic enzymes such as laccase (Lac), manganese peroxidase (MnP), aryl alcohol oxidase (AAO), and lignin peroxidase (LiP) by solid-state fermentation (SSF), which was carried out using a support substrate from the fruit juice industry. The chemical content of grape waste from this industry was studied. Also, the production patterns of these extracellular enzymes were researched during the growth of the organism for a period of 20 days and the protein, reducing sugar, and nitrogen levels were monitored during the stationary cultivation. The highest Lac activity was obtained as 2247.62 +/- 75U/L on day 10 in the presence of 750 mu M Mn2+, while the highest MnP activity was attained as 2198.44 +/- 65U/L on day 15 in the presence of 500 mu M Mn2+. Decolorization of methyl orange and reactive red 2 azo dyes was also achieved with ligninolytic enzymes, produced in SSF of P. eryngii.