INVESTIGATION OF THE EFFECT OF SQUALENE ON CISPLATIN-INDUCED TOXICITYIN KELLY NEUROBLASTOMA CELL LINE


Özdemir ., Gökbayrak Ö., Serinan E. Ö., Aktaş S.

VIII.Turkey in vitro Diagnostic Symposia: Preclinical Models, İzmir, Turkey, 29 November - 01 December 2023, vol.48, no.2, pp.78-79

  • Publication Type: Conference Paper / Summary Text
  • Volume: 48
  • Doi Number: 10.1515/tjb-2023-48s209
  • City: İzmir
  • Country: Turkey
  • Page Numbers: pp.78-79
  • Dokuz Eylül University Affiliated: Yes

Abstract

Objective: It is aimed at investigating the effect of the squalene substance obtained from olive oil and the combinations of squalene-cisplatin on the Kelly neuroblastoma cell line. Materials-Methods:Kelly cells were treated with the different concentrations (200-3,12 uM) of squalene and the combinations of these doses with 50 uM, 20 uM and 10 uM cisplatin for 24, 48 and 72 hours. Cell viability was investigated by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide) cell proliferation assay. The effects of 200 uM squalene - 50 uM cisplatin (CDDP) and 3.12 uM squalene-50 uM cisplatin combinations on apoptotic induction of Kelly cell line were examined by Annexin-V FITC assay and flow cytometry analysis. The effect of these combinations on cell cycle of Kelly cell line was investigated with PI (propidium iodide) and flow cytometry analysis. Results: As a result of cell proliferation tests, cell viability was determined to be above 50% in the tested dose range of squalene at 24, 48 and 72 hours incubation periods. The lowest cell viability at 24 hours was detected in the combination of 50 uM CDDP with 3.12 uM squalene. According to apoptosis results, a statistically significant increase in apoptosis was detected in both the 50 uM CDDP + 200 uM SQ group and the 50 uM CDDP + 3.12 uM SQ group compared to the control group. Although the 50 uM CDDP+200 uM SQ group increased apoptosis compared to the 50 uM CDDP+3.12 uM SQ group, no statistically significant difference was detected between these two groups. Conclusion: Squalene did not show any antitumoral effect on the MYCN(+) neuroblastoma KELLY cell line. The combination of squalene with CDDP did not alter CDDP toxicity in Kelly cells. Keywords: squalene, cisplatin, neuroblastoma