Akademik Veri Yönetim Sistemi
VIII.Turkey in vitro Diagnostic Symposia: Preclinical Models, İzmir, Türkiye, 29 Kasım - 01 Aralık 2023, cilt.48, sa.2, ss.78-79
Objective: It is aimed at investigating the effect of the
squalene substance obtained from olive oil and the
combinations of squalene-cisplatin on the Kelly
neuroblastoma cell line.
Materials-Methods:Kelly cells were treated with the
different concentrations (200-3,12 uM) of squalene and the
combinations of these doses with 50 uM, 20 uM and 10 uM
cisplatin for 24, 48 and 72 hours. Cell viability was
investigated by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-
diphenyltetrazolium bromide) cell proliferation assay. The
effects of 200 uM squalene - 50 uM cisplatin (CDDP) and
3.12 uM squalene-50 uM cisplatin combinations on apoptotic
induction of Kelly cell line were examined by Annexin-V
FITC assay and flow cytometry analysis. The effect of these
combinations on cell cycle of Kelly cell line was investigated
with PI (propidium iodide) and flow cytometry analysis.
Results: As a result of cell proliferation tests, cell viability
was determined to be above 50% in the tested dose range of
squalene at 24, 48 and 72 hours incubation periods. The
lowest cell viability at 24 hours was detected in the
combination of 50 uM CDDP with 3.12 uM squalene.
According to apoptosis results, a statistically significant
increase in apoptosis was detected in both the 50 uM CDDP
+ 200 uM SQ group and the 50 uM CDDP + 3.12 uM SQ
group compared to the control group. Although the 50 uM
CDDP+200 uM SQ group increased apoptosis compared to
the 50 uM CDDP+3.12 uM SQ group, no statistically
significant difference was detected between these two groups.
Conclusion: Squalene did not show any antitumoral effect on
the MYCN(+) neuroblastoma KELLY cell line. The
combination of squalene with CDDP did not alter CDDP
toxicity in Kelly cells.
Keywords: squalene, cisplatin, neuroblastoma