Zymodeme patterns of Entamoeba histolytica strains isolated from clinical patients

Kirdar S., Bahar I., Yulug N.

9th International Congress of Parasitology (ICOPA IX), Chiba, Japan, 24 - 28 August 1998, pp.1093-1097 identifier

  • Publication Type: Conference Paper / Full Text
  • Volume:
  • City: Chiba
  • Country: Japan
  • Page Numbers: pp.1093-1097
  • Dokuz Eylül University Affiliated: No

Abstract

Amebiasis is a common medical problem throughout the world. Recently, the nonpathogenic, genetically distinct and morphologically identical parasite Entamoeba dispar was differentiated from invasive Entamoeba histolytica(1). It has has been shown that isoenzyme analysis of the cultured amebae can be used for differentiation. In this study, we aimed to determine isoenzyme patterns of pathogenic E. histolytica strains. Maleic enzyme ME (E.C.1.1.1.40), hexokinase HK (E.C.2.7.1.1), phosphoglucomutase PGM (E.C.2.7.5.1) and glucosephosphate isomerase GPI (E.C.5.3.1.9) enzyme bands were determined by Laemmli method of polyacrylamide gel electrophoresis in all of 5 E.histolytica stains characterized by direct microscopic examination and trichrome staining.