Akademik Veri Yönetim Sistemi
Annals of Medical Sciences, cilt.6, sa.1, ss.7-11, 1997 (Scopus)
Purpose: To investigate the proliferative activity of normal and metaplastic squamous (SM) epithelia, cervical intraepithelial neoplasia (CIN), and squamous carcinoma of the uterine cervix by proliferating cell nuclear antigen (PCNA) immunostaining relative to the histologic classification. Methods: Immunohistochemical staining was performed by streptavidin biotin method using the antibody for PCNA. Nuclear staining degree was graded semiquantitatively and location was netod. Kolmogorou-Smirnov test was used for statistical analysis. Results: Positive staining was confined to basal cells in normal epithelium, basal and parabasal cells in SM and to the layers in which dysplastic changes had occured in CIN. A statistical difference was observed between CIN and carcinoma groups and the squamous metaplasia and CIN groups. No difference was noted for SM and CIN I, CIN I and CIN II and CIN III, large cell keratinizing and large cell non-keratinizing and large cell non-keratinizing carcinomas. Conclusion: These results suggest that a considerable alteration of biologic behaviour occurs in the progression of carcinogenesis from intraepithelial neoplasia to invasive carcinoma. The application of PCNA immunohistochemistry may be helpful in differentiating immature squamous metaplasia and intraepithelial neoplasia.