SOME KINETIC-PARAMETERS AND INACTIVATION OF CATALASE IMMOBILIZED ON MODIFIED POLYVINYLALCOHOL

TARHAN L.

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, vol.31, no.2, pp.109-117, 1991 (SCI-Expanded, Scopus) identifier identifier

  • Publication Type: Article / Article
  • Volume: 31 Issue: 2
  • Publication Date: 1991
  • Doi Number: 10.1007/bf02921782
  • Journal Name: APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.109-117
  • Keywords: IMMOBILIZED CATALASES, POLYVINYLALCOHOL, KINETIC PARAMETERS, BATCH-TYPE REACTORS, H2O2
  • Dokuz Eylül University Affiliated: No

Abstract

Polyvinylalcohol crosslinked with terephthaldicarboxaldehyde and was modified with 2-amino-4,6-dichloro-s-triazine. Optimum conditions for immobilization of catalase on modified and gelatine-coated modified polyvinylalcohol were investigated. Activity variations with respect to pH, temperature, stability behavior, and K(m)(appl) values were investigated for the native and immobilized catalases. Rate constants for H2O2 decomposition and for inactivation of immobilized catalase were determined using a discontinuous batch-type reactor. The influence of H2O2 concentration on the catalase inactivation was investigated.