Stress Condition Induced by H2O2 Differentially Changes Gene Expression Levels of CaMKII Isoforms in Mesenchymal Stem Cells

Cell Death: Degeneration and Regeneration Symposium-Turkey, İstanbul, Türkiye, 3 - 05 Ekim 2019, cilt.1, sa.16, ss.40

ISBN: 978-605-63544-8-9

OP-16

Stress Condition Induced by H2O2 Differentially Changes Gene Expression Levels of CaMKII

Isoforms in Mesenchymal Stem Cells

Tuğba ŞAN1, Pınar AKAN1, Mehmet Emin GÜNEŞ2

1Dokuz Eylul University, Institute of Health Sciences, Department of Neuroscience, İzmir, Turkey

2İzmir Tepecik Training and Research Hospital, Department of Obstetrics and Gynecology, Izmir,

Turkey

Calcium/Calmodulin Dependent Protein Kinase 2 (CAMKII) is one of the main effector enzymes

involved in calcium signaling in eukaryotic cells and it has a key role on the learning process of the

neuronal tissue. The inhibition of calcium / calmodulin dependent protein kinase 2 can prevent

apoptosis in neuronal cells and may also control stem cell proliferation. The isoforms of CaMKII has

different calcium trapping kinetics and affinity for other protein bindings. In this study, we aim to

investigate the changes on the level of gene expressions of CaMKII isoforms (alpha, beta, gama, delta)

under the stress conditions induced by Hydrogen peroxide (H2O2) in mesenchymal stem cells.

Mesenchymal stem cells were obtained from human umbilical cord by explant method. CaMKII

enzyme isoforms levels were determined by RT-qPCR under H2O2 induced stress conditions. Effects

of application of KN-93 which is a CaMKII inhibitor on stem cell viability and proliferation were

determined by MTT and MTS tests.

It was shown that the application of H2O2 (1mM) for 30 minutes to generate oxidative stress

decreased the cell viability ~40 – 50% in proportion to control group. In WJ-MSCs without any

application, there was no statistically significant difference between the gene expression levels of

alpha, beta, gamma and delta isoforms (p> 0.05). After H2O2 (1mM) treatment gene expression level

of CaMKII Beta isoform increased 7.7 fold compared to the control group but interestingly gene

expression level of CaMKII Delta isoform significantly reduced (p <0.05).

In conclusion, according to our literature review, the relationship between CaMKII isoforms and

mesenchymal stem cell viability and their changes in stress conditions induced by H2O2 were firstly

demonstrated by this study. The data which were obtained from our study may provide a

fundamental ground to understand response mechanism of MSCs to the stress conditions and to

develop targeted stem cell treatment strategies.

Keywords: Mesenchymal Stem Cell, Wharton’s Jelly, Oxidative Stress, CaMKII, KN-93, KN- 92