Cell Death: Degeneration and Regeneration Symposium-Turkey, İstanbul, Turkey, 3 - 05 October 2019, vol.1, no.16, pp.40
ISBN: 978-605-63544-8-9
OP-16
Stress Condition Induced by H2O2 Differentially Changes Gene Expression Levels of CaMKII
Isoforms in Mesenchymal Stem Cells
Tuğba ŞAN1, Pınar AKAN1, Mehmet Emin GÜNEŞ2
1Dokuz Eylul University, Institute of Health Sciences, Department of Neuroscience, İzmir, Turkey
2İzmir Tepecik Training and Research Hospital, Department of Obstetrics and Gynecology, Izmir,
Turkey
Calcium/Calmodulin Dependent Protein Kinase 2 (CAMKII) is one of the main effector enzymes
involved in calcium signaling in eukaryotic cells and it has a key role on the learning process of the
neuronal tissue. The inhibition of calcium / calmodulin dependent protein kinase 2 can prevent
apoptosis in neuronal cells and may also control stem cell proliferation. The isoforms of CaMKII has
different calcium trapping kinetics and affinity for other protein bindings. In this study, we aim to
investigate the changes on the level of gene expressions of CaMKII isoforms (alpha, beta, gama, delta)
under the stress conditions induced by Hydrogen peroxide (H2O2) in mesenchymal stem cells.
Mesenchymal stem cells were obtained from human umbilical cord by explant method. CaMKII
enzyme isoforms levels were determined by RT-qPCR under H2O2 induced stress conditions. Effects
of application of KN-93 which is a CaMKII inhibitor on stem cell viability and proliferation were
determined by MTT and MTS tests.
It was shown that the application of H2O2 (1mM) for 30 minutes to generate oxidative stress
decreased the cell viability ~40 – 50% in proportion to control group. In WJ-MSCs without any
application, there was no statistically significant difference between the gene expression levels of
alpha, beta, gamma and delta isoforms (p> 0.05). After H2O2 (1mM) treatment gene expression level
of CaMKII Beta isoform increased 7.7 fold compared to the control group but interestingly gene
expression level of CaMKII Delta isoform significantly reduced (p <0.05).
In conclusion, according to our literature review, the relationship between CaMKII isoforms and
mesenchymal stem cell viability and their changes in stress conditions induced by H2O2 were firstly
demonstrated by this study. The data which were obtained from our study may provide a
fundamental ground to understand response mechanism of MSCs to the stress conditions and to
develop targeted stem cell treatment strategies.
Keywords: Mesenchymal Stem Cell, Wharton’s Jelly, Oxidative Stress, CaMKII, KN-93, KN- 92