Plant cytosine-5 DNA methyltransferases: Structure, function, and molecular evolution
GENOMICS, cilt.90, sa.4, ss.530-541, 2007 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 90 Sayı: 4
- Basım Tarihi: 2007
- Doi Numarası: 10.1016/j.ygeno.2007.06.011
- Dergi Adı: GENOMICS
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.530-541
- Anahtar Kelimeler: C5-DNA methyltransferase, CMT family, Dnmt2 family, DRM family, METI family, methylation, TRD
- Dokuz Eylül Üniversitesi Adresli: Hayır
Özet
A detailed analysis of the structure and function, along with evolutionary aspects, of the main plant cytosine-5 DNA methyltransferases (C5-MTases) is presented. The evolutionary relationships between the already known and four candidate plant C5-MTases identified in this work were investigated using the distance, maximum-parsimony, and maximum-likelihood approaches. The topologies of the trees were overall congruent: four monophyletic groups corresponding to the four plant C5-MTase families were clearly distinguished. In addition, sequence analyses of the plant C5-MTase target recognition domain sequences were performed and phylogenetic trees were reconstructed showing that there is good conservation among but not within the plant C5-MTase families. Furthermore, a conserved dipeptide that plays an important role in flipping the target base into the catalytic site of the C5-MTases was identified in all plant C5-MTases under study. (C) 2007 Elsevier Inc. All rights reserved.