The effects of adenosine A1 selective agonist on metamidophos-induced cholinergic symptoms and brain oxidative stres in rats


Kalkan Ş., Yılmaz D., Ergür U. B., Uysal Harzadın N., Arıcı M. A., Topçu A., ...More

2007 North American Congress of Clinical Toxicology Annual Meeting, Louisiana, United States Of America, 19 - 24 October 2007, vol.45, no.1, pp.629

  • Publication Type: Conference Paper / Summary Text
  • Volume: 45
  • City: Louisiana
  • Country: United States Of America
  • Page Numbers: pp.629
  • Dokuz Eylül University Affiliated: Yes

Abstract

Background: The aim of the study was to evaluate the effects of different doses of an adenosine A1 selective agonist phenylisopropyl adenosine (PIA), on metamidophos-induced cholinergic symptoms, mortality, diaphragm muscle necrosis, brain antioxidant enzyme activities and thiobarbituric acid reactive substances (TBARS) levels. Methods: Metamidophos 20 mg/kg p.o. followed by 1 ml/kg 0.9 % sodium chloride, 1 mg/kg, 2 mg/kg, 3 mg/kg or 5 mg/kg PIA, i.p. were given to the rats (n = 8 in each group), respectively. Initial times for clinical signs and diaphragma necrosis were compared with Kruskal-Wallis Analysis. Clinical signs between groups were compared with Fisher’s Exact Test. Survival analysis was based on the Kaplan Meier procedure. Mann-Whitney U test was used to compare brain antioxidant enzyme activities and TBARS levels in groups. Results: Initial time of clinical signs including chewing, salivation and convulsion were delayed in the groups that treated with 1 or 5 mg/kg PIA, 1 or 2 mg/kg PIA and 1 mg/kg PIA (p 0.05, p < 0.01, p < 0.01, p < 0.01, p < 0.05), respectively. PIA was effective to reverse the necrotic changes in diaphgram muscle induced by metamidophos significantly in all treatment groups (p < 0.01). Brain TBARS levels were significantly increased after the metamidophos poisoning (p < 0.001). PIA (2 to 5 mg/kg) significantly decreased in brain TBARS levels compared to 0.9 % sodium chloride treated rats (p < 0.001). Discussion: Although different doses of PIA reduced the OP- induced oxidative stress and diaphragm necrosis, a single dose of PIA was not able to recover cholinergic symptoms of metamidophos poisoning. Conclusion: Cumulative or repeated dose regimen of PIA might be more effective to prevent symptoms of metamidophos poisoning in rats.