A novel carrier for Phanerochaete chrysosporium immobilization

Urek R., Pazarlioglu N.

ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY, vol.32, no.4, pp.563-574, 2004 (SCI-Expanded) identifier

  • Publication Type: Article / Article
  • Volume: 32 Issue: 4
  • Publication Date: 2004
  • Doi Number: 10.1081/labb-200039618
  • Journal Name: ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED)
  • Page Numbers: pp.563-574
  • Keywords: adsorption kinetics, carriers and immobilization, manganese peroxidase, Phanerochaete chrysosporium, polystyrene foam, LIGNIN PEROXIDASE PRODUCTION, SOLID-STATE FERMENTATION, POLYURETHANE FOAM, ADHESION, GROWTH, FEED
  • Dokuz Eylül University Affiliated: Yes

Abstract

Phanerochaete chrysosporium BKMF-1767 cells were immobilized on different carriers. The optimum carrier according to adsorbed P. chrysosporium cells number (86.38%) was determined to be polystyrene foam, a novel carrier. The conditions for the immobilization of cells on polystyrene foam were optimized and determined as 50 rpm, 37degreesC, and 2 h. The results show that the adsorption of P. chrysosporium on polystyrene foam follows the Langmuir adsorption isotherm. High manganese peroxidase activity (421 U/L) and dry mass (4.7 g/L) were recovered from the batch mode polystyrene foam solid state fermentation system.