Erythropoietin decreases cytotoxicity and nitric oxide formation induced by inflammatory stimuli in rat oligodendrocytes
PHYSIOLOGICAL RESEARCH, vol.55, no.1, pp.33-38, 2006 (SCI-Expanded, Scopus)
- Publication Type: Article / Article
- Volume: 55 Issue: 1
- Publication Date: 2006
- Doi Number: 10.33549/physiolres.930749
- Journal Name: PHYSIOLOGICAL RESEARCH
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Page Numbers: pp.33-38
- Keywords: erythropoietin, oligodendrocyte, cytotoxicity, lipopolysaccharide, interferon-gamma, nitric oxide
- Dokuz Eylül University Affiliated: Yes
Abstract
In the present study, we investigated whether erythropoietin (Epo) has a protective effect against cytotoxicity induced by interferon-gamma (IFN-gamma) and lipopolysaccharide (LPS) in primary rat oligodendrocyte cultures. The possible modulatory effect of erythropoietin on inducible nitric oxide synthase (iNOS) mRNA expression and nitrite production were also analyzed. Erythropoietin exerted a significant protective effect against IFN-gamma and LPS-induced oligodendrocyte injury as determined by lactate dehydrogenase assay. Treatment with erythropoietin inhibited the expression of iNOS mRNA and nitrite production resulting from proinflammatory stimulation by IFN-gamma and LPS. These results suggest that erythropoietin has protective effects against inflammatory oligodendrocyte injury in vitro and may play a protective role in neurological disorders characterized by oligodendrocyte death, Such as multiple sclerosis.