Distinct Structures and Dynamics of Chromatosomes with Different Human Linker Histone Isoforms
MOLECULAR CELL, cilt.81, sa.1, ss.166-188, 2021 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 81 Sayı: 1
- Basım Tarihi: 2021
- Doi Numarası: 10.1016/j.molcel.2020.10.038
- Dergi Adı: MOLECULAR CELL
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, Chemical Abstracts Core, EMBASE, MEDLINE, DIALNET
- Sayfa Sayıları: ss.166-188
- Dokuz Eylül Üniversitesi Adresli: Evet
Özet
The repeating structural unit of metazoan chromatin is the chromatosome, a nucleosome bound to a linker histone, H1 There are 11 human H1 isoforms with diverse cellular functions, but how they interact with the nucleosome remains elusive, Here, we determined the cryoelectron microscopy (cryo-EM) structures of chromatosomes containing 197 bp DNA and three different human H1 isoforms, respectively. The globular domains of all three H1 isoforms bound to the nucleosome dyad. However, the flanking/linker DNAs displayed substantial distinct dynamic conformations. Nuclear magnetic resonance (NMR) and H1 tail-swapping cryo-EM experiments revealed that the C-terminal tails of the H1 isoforms mainly controlled the flanking DNA orientations. We also observed partial ordering of the core histone H2A C-terminal and H3 N-terminal tails in the chromatosomes. Our results provide insights into the structures and dynamics of the chromatosomes and have implications for the structure and function of chromatin.