rpoB gene mutations in rifampin-resistant Mycobacterium tuberculosis strains isolated in the Aegean region of Turkey

Oguz V., Eroglu C., Guneri S., Yapar N., Oztop A., Sanic A., ...Daha Fazla

JOURNAL OF CHEMOTHERAPY, cilt.16, sa.5, ss.442-445, 2004 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 16 Sayı: 5
  • Basım Tarihi: 2004
  • Doi Numarası: 10.1179/joc.2004.16.5.442
  • Dergi Adı: JOURNAL OF CHEMOTHERAPY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.442-445
  • Anahtar Kelimeler: M. tubercolosis, rifampin, rpoBgene mutations
  • Dokuz Eylül Üniversitesi Adresli: Evet

Özet

Although DNA sequencing method has not been used routinely for detecting resistance of Mycobacterium tuberculosis to antituberculosis drugs, it is suggested for the investigation of gene mutations causing resistance. Using DNA sequencing (Automated Applied Biosystems), we attempted to determine mutations in the 81bp cor region (rifampin resistance determining region) of the rpoB gene in 48 Mycobacterium tuberculosis strains found to be rifampin resistant by classical phenotypic methods. Of the 48 strains, 46 (95.8%) were found to have rpo gene mutations with 13 different types while in two (4.2%) of the 48 strains, no mutations were detected. None of the strains had mutations at more than one codon. Point mutations at the 531st (52.1 %) and 526th (18.9 %) codons were frequent. The most frequent point mutation was Ser531Leu, and it was found in 21 (4398 %) of 48 strains. This is the first study from Turkey, reporting Ser522Leu point mutation in one isolate and deletion of 515th codon (ATG - Met) in two isolates.