Fermentation strategies for recombinant protein expression in the methylotrophic yeast Pichia pastoris

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Zhang W., İNAN M., Meagher M. M.

Biotechnology and Bioprocess Engineering, cilt.5, sa.4, ss.275-287, 2000 (Scopus) identifier

  • Yayın Türü: Makale / Derleme
  • Cilt numarası: 5 Sayı: 4
  • Basım Tarihi: 2000
  • Doi Numarası: 10.1007/bf02942184
  • Dergi Adı: Biotechnology and Bioprocess Engineering
  • Derginin Tarandığı İndeksler: Scopus
  • Sayfa Sayıları: ss.275-287
  • Anahtar Kelimeler: Fed-batch growth, Fermentation optimization, Growth modeling, Methylotrophic yeast, Pichia pastoris, Recombinant protein expression
  • Dokuz Eylül Üniversitesi Adresli: Hayır

Özet

Fermentation strategies for recombinant protein production in Pichia pastoris have been investigated and are reviewed here. Characteristics of the expression system, such as phenotypes and carbon utilization, are summarized. Recently reported results such as growth model establishment, application of a methanol sensor, optimization of substrate feeding strategy, DOstat controller design, mixed feed technology, and perfusion and continuous culture are discussed in detail.