Akademik Veri Yönetim Sistemi
TURKISH JOURNAL OF UROLOGY, cilt.31, sa.1, ss.17-20, 2005 (ESCI)
Introduction: Dimethyl Sulphoxide (DMSO), with exceptional solvent properties, is used in various pharmacological studies on corpus cavernosum as a vehicle. However, there is little known regarding the relaxing characteristics of this solvent. Thus we aimed to determine the highest noneffecious concentration of DMSO as a solvent in isolated human corpus cavernosum (HCC). Materials and Methods: Strips (3x3x7 mm) were cut longitudinally from human corpus cavernosum (HCC) tissues and mounted in 10 ml organ bath with the Krebs solution. The strips were oxygenated with 95% O-2 and 5% CO2 and the temperature was maintained at 37 degrees C. HCC tissues were contracted with 3x10(-6) M phenylephrine and relaxation responses were evaluated by cumulative (10(-9) - 10(-5) M) and (10(-12) - 10(-9) M) addition of DMSO. Results: Cumulative application of phenylephrine (10(-8) - 10(-5) M) caused concentration dependent contraction response in HCC tissue (pD(2) 6.24 +/- 0.14). DMSO (10(-9) - 10(-5) M) had dose dependent relaxing effects on precontracted HCC tissues with phenylephrine (3x10(-6) M). HCC tissues exhibited relaxation response to DMSO 29 +/- 5.4% of phenylephrine-induced tone (IC50 6.63 +/- 0.4). At lower concentration (10(-1)2 - 10(-9) M) DMSO did not cause relaxation response curve. Conclusion: In this study we demonstrated that DMSO could be used in isolated HCC studies as a solvent at concentrations lower than 10(-9) M.