Akademik Veri Yönetim Sistemi
12th Quantitative Phase Imaging, California, Amerika Birleşik Devletleri, 17 - 19 Ocak 2026, cilt.13861, (Tam Metin Bildiri)
Digital off-Axis holographic microscopy (DHM) is an interferometric quantitative phase imaging (QPI) technique that enables label-free, single-shot imaging of biological specimens with high temporal bandwidth. However, the achievable space-bandwidth product (SBP) in DHM is inherently limited by the requirement to spatially isolate the signal term in the Fourier domain, which restricts the recovery of high spatial frequencies. To address this limitation, we integrate the fractional Fourier transform (FrFT) into both the hologram recording and filtering stages, replacing the conventional Fourier transform-based workflow. The FrFT generalizes the Fourier transform through a continuous fractional order parameter, enabling controlled redistribution of spatial-frequency content between the spatial and frequency domains. By combining optical and digital FrFT operations in a hybrid FrFT-DHM framework, we engineer an asymmetric spatialfrequency distribution in which the signal term is more efficiently separated from the direct current (DC) and twin-image components. This hybrid configuration improves utilization of the sensor's available spatial bandwidth, enabling enhanced recovery of high-frequency information without increasing acquisition time or compromising temporal performance. We experimentally demonstrate the proposed approach through label-free phase imaging of live adherent epithelial cells. Compared to conventional Fourier-based DHM, the hybrid FrFT-DHM method provides improved spatial resolution and reveals finer morphological details in cellular structures. These results highlight the potential of hybrid FrFT-based DHM as a practical and effective QPI modality for high-resolution, dynamic live-cell imaging applications.