Hemodynamic Effects of Levosimendan on Dexmedetomidine Infusion in Rats Under Isoflurane Anesthesia

Caglar T., HEPAĞUŞLAR H., Girgin A. P., Ustaoglu A., YILMAZ O., Elar Z.

TURKIYE KLINIKLERI TIP BILIMLERI DERGISI, vol.32, no.1, pp.39-46, 2012 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 32 Issue: 1
  • Publication Date: 2012
  • Doi Number: 10.5336/medsci.2010-21549
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.39-46
  • Keywords: Isoflurane, dexmedetomidine, simendan, heart rate, blood pressure, rats, MINIMUM ALVEOLAR CONCENTRATION, SURGICAL-PATIENTS, EFFICACY, DOGS
  • Dokuz Eylül University Affiliated: Yes


Objective: In this study, hemodynamic effects of levosimendan (L) on dexmedetomidine (D) infusion in rats were investigated under isoflurane (I) anesthesia. Material and Methods: A total of eighteen female Wistar-Albino rats were divided into 3 groups. Rats were artificially respirated following cannulation and tracheostomy. At the end of the stabilization period (15 min), initial hemodynamic values were recorded and (i) 1(1.4%) was administered to Group I; (ii) 1(1.4%) and D (1 mu g/kg/dk) were administered to Group ID and (iii) 1 (1.4%), D (1 mu g/kg/dk) and L (0.3 mu g/kg/dk) were administered to Group IDL for 60 min. Heart rate (HR)(bpm), systolic (SAP), diastolic (DAP) and mean arterial pressure (MAP) (mmHg) were recorded with 5 min intervals were and given as mean and/or %. One way variance analysis, Tukey-Kramer and paired t tests were used, p<0.05 was considered the level of significance. Results: No difference was found between groups in terms of initial hemodynamic values. On the contrary, significant differences were found in HR (p=0.03) and DAP (p=0.03) at the end of the infusion period between groups. The differences were correlated to the lower HR in Group ID (176) compared to Group 1(237), (p<0.05) and a higher DAP in Group IDL (67) compared to Group 1(48), (p<0.05). In in-group comparisons; SAP (80 vs. 71 [90%], p=0.03), DAP (59 vs. 48 [81%] p=0.049), MAP (69 vs. 57 [83%], p=0.03) decreased significantly in Group I and HR (206 vs. 176 [85%], p=0.008) decreased significantly in Group ID at the end of the infusion period when compared to baseline values. Conclusion: In rats, I led to a decrease in systemic arterial pressure; and Din the presence of I led to a decrease in HR. Isoflurane-related systemic arterial pressure decrease was prevented by using of D and combination of D and L. Additionally, D-related HR decrease in the presence of I was prevented by using L. In this study, positive effects of D and DL utilization were observed when systemic arterial pressure dropped due to I. Besides, positive effect of L utilization was observed when HR decreased due to D in the presence of I in rats.