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TBS International Biochemistry Congress 2022, 33rd National Biochemsitry Congress, İzmir, Turkey, 26 - 30 October 2022, vol.47, no.2, pp.110, (Summary Text)
BACKGROUND AND AIM: Anaplastic Thyroid Cancer (ATK);
It is one of the aggressive malignancies whose potential molecular
mechanisms have not been elucidated and is the 9th among common cancers. It is resistant to existing cytostatic agents.To support
the current therapy, the antimetastatic property of Tetrahydrocurcumin (THC) was compared with DX and in-vitro studies were
conducted to demonstrate synergism in combination use.
MATERIALS and METHODS: IC25 and IC50 concentrations of
THC and DX in CAL62 and 8505C cell lines were determined by
the modified MTT method we developed. Cell motility, indicative of metastatic potential; In-vitro wound healing method and 3D
colony formation potential were evaluated with Agarose Gel Spheroid Model. The effects of treatments on oxidation on cells were
evaluated by TAS and TOS analyzes. SPSS program was used in
the evaluation. Compliance with normal distribution (Kolmogorov-Smirnov/Shapiro-Wilk tests), comparison of groups was evaluated with ANOVA, Significance, Holm-Sidak test.
RESULTS: In migration analysis; It was observed that the THC50
dose decreased the migration significantly compared to the DX50
and DX+THC (Combined) groups (P<0.05). DX+THC; It was observed that it reduced spheroid formation the most, and there was a
close effect between this dose effect and the THC25 dose in terms
of spheroid formation and numbers (P<0.05). There was no statistically significant difference in Total Oxidant level between the
combination and control.
CONCLUSION: We predict that THC may be effective in metastasis and tumor growth at low doses in ATK cells. We think that in
combined use, THC reduces the oxidative stress of DX.
Keywords: oxidative stress, antineoplastic, Migration