Akademik Veri Yönetim Sistemi
Jordan Journal of Biological Sciences, cilt.14, sa.5, ss.1035-1043, 2021 (Scopus)
In embryonic stem cells, E-cadherin plays a crucial role in sustaining the pluripotent state; this is achieved by the simultaneous presence of active core pluripotency transcriptional network, proper cell-cell adhesion, and an undifferentiated-state chromatin signature. In contrast, N-cadherin is linked to a more differentiated cell state but can support pluripotency if expressed as a knock-in allele from the E-cadherin locus. This study describes the N-cadherin ki/ki embryonic stem cells, which lack E-cadherin expression, to identify transcriptional changes distinct from the known changes observed in E-cadherin knockout embryonic stem cells. As a result, a remarkable similarity in the expression profiles of N-cadherin ki/ki and wild-type embryonic stem cells was observed. Further analysis of the slight differences revealed significant alterations in several biological processes such as chromatin organization and epithelial-mesenchymal transition. The findings presented here shed light on a new aspect of E-cadherin biology in embryonic stem cells and lay the foundations for comprehensive understanding of E-cadherin's functional relevance beyond the prominent role in maintaining pluripotency.