Akademik Veri Yönetim Sistemi
TURKIYE KLINIKLERI TIP BILIMLERI DERGISI, cilt.28, sa.5, ss.704-714, 2008 (SCI-Expanded)
In the Fourier Transform Infrared (FTIR) spectroscopy technique, the absorption of infrared (IR) radiation, which is known to be a harmless electomagnetic wave to the cellular components, causes Specific molecular vibrations. As a result, characteristic signals (bands) appear at particular frequency values. The changes in the Intensity, bandwidth and frequency values of these bands give valuable information at molecular level on the investigated system. The combination of a microscope with a FTIR spectrometer results in FTIR microspectroscopy, which has a high spatial and spectral resolution. FTIR spectroscopy has been used in many different fields ranging from astronomy to chemistry for many years. In the last years, in parallel with the rapid advances in microspectroscopy technology. the number of its application areas increased remarkably. The advances in the application of FTIR microspectroscopy in medicine occured particulary, in pathology, clinical biochemistry and IR imaging. This technique, which enables LIS to obtain point-by-point biochemical map using a thin tissue slide having thickness in pin values, can be used to have information about the biochemical changes induced by many diseases. In addition, this technique can also be used for diagnostic purposes. Since there is no need for any kind of staining, extraction or purification procedures in this microspectroscopic technique, the investigated system remains intact in terms of its molecular structure. In this review, general properties of electomagnetic waves, the basic principles of FTIR microspectroscopy and its experimental preparation methods were Summarized and its main applications in the medical field were reviewed.