A Long Fluorescence Lifetime Probe for Labeling of Gram-Negative Bacteria

Baibek A., Konieczna Z., ÜÇÜNCÜ M., Alghamdi Z. S., Sharma R., Horrocks M. H., ...Daha Fazla

CHEMICAL & BIOMEDICAL IMAGING, cilt.3, ss.45-50, 2025 (ESCI) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 3
  • Basım Tarihi: 2025
  • Doi Numarası: 10.1021/cbmi.4c00066
  • Dergi Adı: CHEMICAL & BIOMEDICAL IMAGING
  • Derginin Tarandığı İndeksler: Emerging Sources Citation Index (ESCI), Scopus
  • Sayfa Sayıları: ss.45-50
  • Anahtar Kelimeler: fluorescence lifetime imaging, bacteria targeting, bacterial detection, optical imaging, fluorescentprobe, polymyxin probe
  • Dokuz Eylül Üniversitesi Adresli: Hayır

Özet

Bacterial resistance, primarily stemming from misdiagnosis, misuse, and overuse of antibacterial medications in humans and animals, is a pressing issue. To address this, we focused on developing a fluorescent probe for the detection of bacteria, with a unique feature-an exceptionally long fluorescence lifetime, to overcome autofluorescence limitations in biological samples. The polymyxin-based probe (ADOTA-PMX) selectively targets Gram-negative bacteria and used the red-emitting fluorophore azadioxatriangulenium (with a reported fluorescence lifetime of 19.5 ns). Evaluation of ADOTA-PMX's bacterial labeling efficacy revealed strong specificity for Gram-negative bacteria, and full spectral fluorescence lifetime imaging microscopy demonstrated the potential of ADOTA-PMX for bacterial imaging applications. The probe exhibited a lifetime of 4.5 ns when bound to bacteria, possibly indicating interactions with the bacterial outer membrane. Furthermore, the fluorescence lifetime measurements of ADOTA-PMX labeled bacteria could be performed using a benchtop fluorimeter without the need of sophisticated microscopes. This study represents the first targeted probe for fluorescence lifetime imaging, offering sensitivity for detecting Gram-negative bacteria and enabling multiplexing via fluorescence lifetime imaging.