Akademik Veri Yönetim Sistemi
SCIENCE, cilt.345, sa.6198, ss.785-793, 2014 (SCI-Expanded, Scopus)
Adverse prenatal environments can promote metabolic disease in offspring and subsequent generations. Animal models and epidemiological data implicate epigenetic inheritance, but the mechanisms remain unknown. In an intergenerational developmental programming model affecting F-2 mouse metabolism, we demonstrate that the in utero nutritional environment of F-1 embryos alters the germline DNA methylome of F-1 adult males in a locus-specific manner. Differentially methylated regions are hypomethylated and enriched in nucleosome-retaining regions. A substantial fraction is resistant to early embryo methylation reprogramming, which may have an impact on F-2 development. Differential methylation is not maintained in F-2 tissues, yet locus-specific expression is perturbed. Thus, in utero nutritional exposures during critical windows of germ cell development can impact the male germline methylome, associated with metabolic disease in offspring.