A Bio-Acoustic Levitational (BAL) Assembly Method for Engineering of Multilayered, 3D Brain-Like Constructs, Using Human Embryonic Stem Cell Derived Neuro-Progenitors

Bouyer C., Chen P., GÜVEN S., DEMİRTAŞ T. T., Nieland T. J. F., Padilla F., ...More

Advanced Materials, vol.28, no.1, pp.161-167, 2016 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 28 Issue: 1
  • Publication Date: 2016
  • Doi Number: 10.1002/adma.201503916
  • Journal Name: Advanced Materials
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.161-167
  • Keywords: bulk acoustic standing waves, multilayered 3D tissue structures, neural constructs, neural progenitor cells, tissue engineering
  • Dokuz Eylül University Affiliated: Yes

Abstract

© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.A bio-acoustic levitational assembly method for engineering of multilayered, 3D brainlike constructs is presented. Acoustic radiation forces are used to levitate neuroprogenitors derived from human embryonic stem cells in 3D multilayered fibrin tissue constructs. The neuro-progenitor cells are subsequently differentiated in neural cells, resulting in a 3D neuronal construct with inter and intralayer neurite elongations.