Common mutations at the homocysteine metabolism pathway and pediatric stroke

Akar N., Akar E., ÖZEL DEMİRALP F. D., Deda G., Sipahi T.

THROMBOSIS RESEARCH, vol.102, no.2, pp.115-120, 2001 (SCI-Expanded) identifier identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 102 Issue: 2
  • Publication Date: 2001
  • Doi Number: 10.1016/s0049-3848(01)00226-2
  • Journal Name: THROMBOSIS RESEARCH
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.115-120
  • Keywords: pediatric stroke, factor V gene, prothrombin gene, methylene tetrahydrofolate reductase, methylene tetrahydrofolate dehydrogenase, methionine synthase reductase, ACTIVATED PROTEIN-C, NEURAL-TUBE DEFECTS, FACTOR-V, RISK FACTOR, METHYLENETETRAHYDROFOLATE REDUCTASE, TURKISH POPULATION, VENOUS THROMBOSIS, CEREBRAL INFARCT, PROTHROMBIN GENE, VASCULAR-DISEASE
  • Dokuz Eylül University Affiliated: No

Abstract

Heterozygosity and/or homozygosity for mutations at the genes of the enzymes involved in homocysteine metabolism may confer an increased risk for thrombosis by causing hyperhomocysteinemia. Although the mutations related to homocysteine metabolism possibly increase the risk of stroke, the data are conflicting and there are very few reports linking these defects to acute stroke in children. We aimed to study the role of these mutations in Turkish children with ischemic stroke. Forty-six patients having cerebral infarct were clinically diagnosed, and the infarction verified with magnetic resonance imaging of the brain was included in the study. All patients were below the age of 18 (10 months to 18 years). Sixty-eight controls, consecutively selected among healthy unrelated subjects from the same geographic area of Turkey without personal and family history of thrombosis, stroke or Behest's disease, were included. Genotyping for the common mutations was carried out by the methods described previously. There was no difference between the pediatric stroke patients and controls for the distribution of methylene tetrahydrofolate reductase (MTHFR) 677 C-T, MTHFR 1298 A-C, methylene tetrahydrofolate dehydrogenase (MTHFD) 1958 G-A and methionine synthase reductase (MTRR) 66 A-G alleles. There was no risk for double alterations (MTHFR 677 C-T vs. 1298 A-C) after individuals with FV 1691 A mutation is excluded. Twelve of the 46 patients were found to carry FV 1691 A mutation (26.0%), one being homozygote. The cerebral infarct risk for FV 1691 A was found to be 6.4 (CI 95% 1.7-23.0). Eight of the 46 patients were found to carry PT 20210 A mutation (16.6%). Two of the FV 1691 A heterozygous patients carried PT 20210 A mutation at the same time (4.2%). As a conclusion, we can say that FV 1691 A and PT 20210 A mutations are important and must be included to the routine analysis of pediatric stroke patients. (C) 2001 Elsevier Science Ltd. All rights reserved.