Akademik Veri Yönetim Sistemi
FASEB Journal, cilt.11, sa.9, 1997 (SCI-Expanded)
This investigation was conducted to elucidate the free radical induced lysosomal membrane injuries in the brain ischemia-reperfusion period. [;sing two (lifik, rent: penumbral (mild) and severe ischemia-reperfusion models, performed on 8.1 Sprague-I)awley rat brains, changes in the total activities and the ratios of cytosolic fraction to the lysosomal fraction (CF/LF) for lysosomal enzymes (CL. AP) were assessed in brain tissues, by spectrophotometric methods. Severe ischemia-reperfusion caused significant increase in total activities and CF/LF ratios for CL compared to the sham group (p0.01), while there were no significant differences between the sham and the penumbral ischemia-reperfiision groups. Statistically significant increases were observed in the ('F/I,i" ratio for AP in severe ischemia-reperfusion groups compared to the sham group (p0.05), while no significant differences were found between the sham and all reperfusion groups for both types of ischemia considering the total AP activities. Our data demonstrate that; although severe ischemia -reperfusion causes redistribution of lysosomal enzymes from lysosonws to cytoplasms and an increase in ('L activities, there are no changes in the lysosomal membrane stability and lysosomal enzyme activities in penumbral ischemiaroper fusion.