Rapid Fibroblast Cell Culture Characterization with Impedimetric Label-free Heat Shock Protein-47 Biosensor

Harmanci D., UYGUN Z. O., Sezgin A. K., DEMİRDÖVER C., GİRGİN SAĞIN F., Akdogan G.

ELECTROANALYSIS, cilt.32, sa.10, ss.2310-2315, 2020 (SCI-Expanded, Scopus) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 32 Sayı: 10
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1002/elan.202060264
  • Dergi Adı: ELECTROANALYSIS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Applied Science & Technology Source, BIOSIS, Chimica, Communication Abstracts, Metadex, Civil Engineering Abstracts
  • Sayfa Sayıları: ss.2310-2315
  • Anahtar Kelimeler: HSP-47, impedance, gold nanoparticle, graphene, chronoimpedance
  • Dokuz Eylül Üniversitesi Adresli: Evet

Özet

In this study, for the first time in literature, an impedimetric biosensor technology was developed to determine Heat Shock Protein-47 (HSP-47) for the characterization of primary skin fibroblast cells. Gold nanoparticle electrodes (GNPE) were modified with amino-functionalized graphene oxide layers and anti-HSP-47 were immobilized on this layer. The performance of the biosensor was tested with real samples as cell lysates. The low detection limit (LOD) and the linear detection limit (LOQ) of the biosensor were calculated as 9.47 pg/mL and 3.23 pg/mL, respectively. Linear measurement range was obtained between 10-160 pg/mL HSP-47. The reproducibility of the biosensor was good.