Purpose: Human papillomavirus (HPV) is the primary cause of cervical cancer. Our study aims to evaluate the effects of vitamin D on HPV regression, related cytological pathologies and various metabolic parameters. Materials and methods: 100 high-risk HPV positive patients, who were detected from hospital database, were divided into 2 groups as those who received vitamin D supplements (n = 50) and did not receive vitamin D supplements (n = 50). We determined the changes in serum vitamin D concentrations, metabolic parameters of patients and regression of HPV after 6 months. Then, we compared the results of patients who took vitamin D supplements, and those who did not. Results: Patient characteristics of both groups were similar in terms of smoking, using oral contraceptives, operation status, body mass index, and mean age of first coitus. Considering the findings of our study, the difference in rate of HPV regression was not significant in the group with a history of vitamin D use (P = 0.804). Regarding metabolic parameters, we observed significant decrease in insulin (P < 0.001), triglyceride (P = 0.019), and CRP (P < 0.001) levels in the group with a history of vitamin D use. Conclusion: According to our findings, vitamin D supplementation does not significantly increase the rate of HPV regression. On the other hand, its metabolic effects are noteworthy. It has positive effects on glucose homeostasis and lipid profile. In the light of previous studies, vitamin D may be helpful in the treatment of vaginal infections, nonetheless it is not a as effective concerning cervical HPV infections.