Evaluation of I-131-Pentamidine for scintigraphy of experimentally Leishmania tropica-infected hamsters


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İnceboz T., Lambrecht F. Y., Eren M. S., Girginkardeşler N., Bekiş R., Yılmaz O., ...More

JOURNAL OF DRUG TARGETING, vol.22, no.5, pp.416-420, 2014 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 22 Issue: 5
  • Publication Date: 2014
  • Doi Number: 10.3109/1061186x.2013.878943
  • Journal Name: JOURNAL OF DRUG TARGETING
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.416-420
  • Keywords: Amastigote, I-131-Pentamidine scintigraphy, Leishmania tropica, Leishmaniasis hamster, promastigote, CUTANEOUS LEISHMANIASIS, PENTAMIDINE, RATS, BIODISTRIBUTION, LESIONS
  • Dokuz Eylül University Affiliated: Yes

Abstract

We aimed to assess the ability of I-131-Pentamidine scintigraphy to detect the lesions of Leishmania tropica infection. An experimental model of cutaneous leishmaniasis was developed. The presence of cutaneous leishmaniasis was confirmed. Pentamidine was radioiodinated with I-131. The radiolabeled pentamidine was validated by the requisite quality control tests to check its radiolabeling efficiency, in vitro stability. I-131-Pentamidine (activity: 18.5 MBq/100 mu l) was injected intracardiacally into infected hamsters. Static whole body images of the hamsters were acquired under the gamma camera at 5 and 30 min, 2, 6 and 24 h following the administration. On the scintigrams, anatomically adjusted regions of interest (ROIs) were drawn over the right feet (target) and left feet (not-target) and various organs. Accumulation of I-131-Pentamidine at sites of infection is expressed as the target to non-target (T/NT) ratio. The results T/NT ratio decreased with time. In concluding the I-131-Pentamidine has poor sensitivity in detection of L. tropica infection.