Sol-gel encapsulated glucose oxidase arrays based on a pH sensitive fluorescent dye

Yildirim N., ODACI DEMİRKOL D., Ozturk G., ALP S., Ergun Y., Dornbusch K., ...More

DYES AND PIGMENTS, vol.89, no.2, pp.144-148, 2011 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 89 Issue: 2
  • Publication Date: 2011
  • Doi Number: 10.1016/j.dyepig.2010.10.003
  • Journal Name: DYES AND PIGMENTS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.144-148
  • Keywords: Fluorescent microscopy, Glucose sensing, Glucose oxidase, 5-oxazolone, Sol-gel, Chitosan, BIOSENSORS
  • Dokuz Eylül University Affiliated: Yes

Abstract

Optical glucose oxidase (GOx) arrays based on pH sensitive fluorescent dye (2-(4-tolyl)-4-[4-(1,4,7,10-tetraoxa-13-azacyclopentadecyl)benzylidene]-5-oxazolone) (CPO) has been constructed. The arrays were prepared by spotting of CPO and GOx together with tetraethoxysilane (TEOS)/Chitosan (CHIT) mixture via a microarrayer. After optimization studies, analytical characterization of enzyme arrays were carried out. The fluorescence intensity of the system was linearly correlated to glucose concentration in the range of 1.0-30.0 mM (in potassium phosphate buffer; 2.5 mM at pH 7.0). Furthermore, the developed arrays were used to analyze glucose in some beverages and HPLC was used as a reference method for independent glucose analysis. (C) 2010 Elsevier Ltd. All rights reserved.