Protective Effect of an Adenosine A(1) Receptor Agonist Against Metamidophos-Induced Toxicity and Brain Oxidative Stress

Kalkan Ş., Çelik Yılmaz D., Ergur B. U., Harzadın N. U., Arıcı M. A., Topcu A., ...More

TOXICOLOGY MECHANISMS AND METHODS, vol.19, no.2, pp.148-153, 2009 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 19 Issue: 2
  • Publication Date: 2009
  • Doi Number: 10.1080/15376510802355141
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.148-153
  • Keywords: Organophosphate (OP), Oxidative Stress, Phenylisopropyl Adenosine (PIA), LIPID-PEROXIDATION, RAT MODEL, INHIBITION, EFFICACY
  • Dokuz Eylül University Affiliated: Yes


The aim of this study was to evaluate the effects of different doses of an adenosine A(1) selective agonist, phenylisopropyl adenosine (PIA), on metamidophos-induced cholinergic symptoms, mortality, diaphragm muscle necrosis, and brain oxidative stress. A LD50 dose of metamidophos (20 mg/kg body weight, p.o.) was followed by 1 mL/kg body weight of 0.9% NaCl or 1 mg/kg, 2 mg/kg, 3 mg/kg, or 5 mg/kg body weight PIA ip. Incidence of clinical signs including chewing, salivation, convulsion, and respiratory distress did not show any significant difference among all treatment groups (p > 0.05). PIA was found to be effective to reverse the necrotic changes in diaphragm muscle induced by metamidophos significantly in all groups. Brain Thiobarbituric Acid Reactive Substance (TBARS) levels were significantly increased after the metamidophos poisoning. Administration of 2 to 5 mg/kg body weight PIA decreased brain TBARS levels compared to 0.9% NaCl treated rats. The results indicate that, although different doses of PIA reduced the OP-induced oxidative stress and diaphragm necrosis, a single dose of PIA was not able to recover cholinergic signs and symptoms of metamidophos poisoning.